Submission historical past
Acquired: 16 February 2021
Accepted: 14 January 2022
Revealed in print: 11 February 2022
Acknowledgments
We thank M. Rosnach for pictures and illustrations.
Funding: this work was funded by the Harvard Paulson College of Engineering and Utilized Sciences, the Wyss Institute for Biologically Impressed Engineering, Nationwide Institutes of Well being Nationwide Heart for Advancing Translational Sciences grant UH3TR000522, and Nationwide Science Basis Supplies Analysis Science and Engineering Heart grant DMR-1420570. Ok.Ok.P. was sponsored by Nationwide Institutes of Well being Nationwide Heart for Advancing Translational Sciences grant 1-UG3-HL-141798-01. S.-J.P. was funded by the Georgia Institute of Expertise and Emory College College of Drugs. G.V.L. was funded by the Workplace of Naval Analysis (T. McKenna, Program Supervisor, ONR 341), grant N00014-15-1-2234, and the Nationwide Science Basis, grant 1830881. H.A.M.A. wish to thank the American Chemical Society for his or her beneficiant assist via the Irving S. Sigal Postdoctoral Fellowship. The views and conclusions contained on this doc are these of the authors and shouldn’t be interpreted as representing the official insurance policies, both expressed or implied, of the Nationwide Institutes of Well being, the Nationwide Science Basis, or the US Authorities. This work was carried out partially on the Harvard Heart for Nanoscale Methods, a member of the Nationwide Nanotechnology Infrastructure Community, which is supported by the Nationwide Science Basis beneath award ECS-0335765. The Heart for Nanoscale Methods is a part of Harvard College.
Writer contributions: Ok.Y.L. and S.-J.P. conceived and designed the research, developed a geometrically insulated cardiac tissue node-integrated muscular bilayer assemble, designed and carried out efficiency experiments, analyzed information, organized figures, and wrote the paper. Ok.Ok.P. conceived and designed the research, developed the concept of a geometrically insulated cardiac tissue node and muscular bilayer, and supervised the mission. A.G.Ok. and G.V.L. contributed to the idea of a geometrically insulated cardiac tissue node and muscular bilayer, respectively. D.M. and G.V.L. contributed to the PIV experiments of each biohybrid and wild-type fish. S.L.Ok. designed optogenetic instruments and edited the manuscript. C.M. assisted the fabrication of the biohybrid fish. S.L.Ok., J.Z., and H.A.M.A. carried out major neonatal rat ventricular harvest for the biohybrid fish optimization. All authors contributed to the preparation of the manuscript;
Competing pursuits: Ok.Y.L., S.-J.P., A.G.Ok., V.T., and Ok.Ok.P. are inventors on a patent filed by Harvard College, U.S. Provisional Patent Software No. 63/299,920, based mostly on the outcomes described on this manuscript. The remaining authors declare no competing pursuits.
