Neuroprotective effects on microglia and insights into the structure-activity relationship of an antioxidant peptide isolated from Pelophylax perezi

J Cell Mol Med. 2022 Apr 23. doi: 10.1111/jcmm.17292. On-line forward of print.

ABSTRACT

Tryptophyllins represent a heterogeneous group of peptides which are one of many first lessons of peptides recognized from amphibian’s pores and skin secretions. Right here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, remoted from the Iberian inexperienced frog Pelophylax perezi. The pores and skin secretion of P. perezi was obtained by electrical stimulation and fractionated utilizing RP-HPLC. De novo peptide sequencing was performed utilizing MALDI MS/MS. The first construction of PpT-2 (FPWLLS-NH₂ ) was confirmed by Edman degradation and subsequently investigated utilizing in silico instruments. PpT-2 shared physicochemical properties with different well-known antioxidants. To check PpT-2 for antioxidant exercise in vitro, the peptide was synthesized by stable part and assessed within the chemical-based ABTS and DPPH scavenging assays. Then, a circulation cytometry experiment was performed to evaluate PpT-2 antioxidant exercise in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the technology of reactive species in PMA-stimulated BV-2 microglia cells. We additional explored potential bioactivities of PpT-2 in opposition to prostate most cancers cells and micro organism, in opposition to which the peptide exerted a reasonable antiproliferative impact and negligible antimicrobial exercise. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells handled with as much as 512 µg/ml and in G. mellonella handled with as much as 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological functions, primarily for the remedy/prevention of neurodegenerative issues.

PMID:35460166 | DOI:10.1111/jcmm.17292